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21 lines
638 B
Plaintext
21 lines
638 B
Plaintext
Optimise combinations of primers and minimise the formation of dimers in
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multiplexed PCR.
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Primer Pooler can:
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* Check through each proposed pool for combinations that are likely to form
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dimers
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* Automatically move prospective amplicons between proposed pools to reduce
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dimer formation
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* Automatically search the genome sequence to find which amplicons overlap, and
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place their corresponding primers in separate pools
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* Optionally keep pool sizes within a specified range
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* Handle thousands of primers without being slow (useful for high-throughput
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sequencing applications)
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* Do all of the above with degenerate primers too.
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